› 1o8c

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF E. COLI K-12 YHDH WITH BOUND NADPH

Released:
DOI: 10.2210/pdb1o8c/pdb
PDB entry 1o8c coloured by chain, front view.
PDB entry 1o8c coloured by chain, side view.
PDB entry 1o8c coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of Escherichia coli YhdH, a putative quinone oxidoreductase.
Sulzenbacher G, Roig-Zamboni V, Pagot F, Grisel S, Salomoni A, Valencia C, Campanacci V, Vincentelli R, Tegoni M, Eklund H, Cambillau C
Acta Crystallogr D Biol Crystallogr 60 1855-62 (2004)
PMID: 15388933

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + NADP(+) = acrylyl-CoA + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable acrylyl-CoA reductase AcuI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 345 amino acids
Theoretical weight: 37.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26646 (Residues: 1-324; Coverage: 100%)
Gene names: JW3222, acuI, b3253, yhdH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P61
Unit cell:
a: 189.313Å b: 189.313Å c: 98.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

10 citation in other articles

Respiration of Escherichia coli can be fully uncoupled via the nonelectrogenic terminal cytochrome bd-II oxidase.
Bekker et al. (2009)
9 more

1 mention without citation

Bayesian refinement of protein functional site matching.
Mardia et al. (2007)