› 1o8p

X-ray diffraction
2Å resolution

Unbound structure of CsCBM6-3 from Clostridium stercorarium

Released:
DOI: 10.2210/pdb1o8p/pdb
PDB entry 1o8p coloured by chain, front view.
PDB entry 1o8p coloured by chain, side view.
PDB entry 1o8p coloured by chain, top view.
Source organism: Thermoclostridium stercorarium
Primary publication:
Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains.
Boraston AB, Notenboom V, Warren RA, Kilburn DG, Rose DR, Davies G
J Mol Biol 327 659-69 (2003)
PMID: 12634060

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 15.52 KDa
Source organism: Thermoclostridium stercorarium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GJ44 (Residues: 507-651; Coverage: 23%)
Gene name: xynA
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 98.645Å b: 98.645Å c: 27.704Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.161 0.203
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
5 more