› 1oa7

X-ray diffraction
2Å resolution

Structure of Melanocarpus albomyces endoglucanase in complex with cellobiose

Released:
DOI: 10.2210/pdb1oa7/pdb
PDB entry 1oa7 coloured by chain, front view.
PDB entry 1oa7 coloured by chain, side view.
PDB entry 1oa7 coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
Crystal structure of a family 45 endoglucanase from Melanocarpus albomyces: mechanistic implications based on the free and cellobiose-bound forms.
Hirvonen M, Papageorgiou AC
J Mol Biol 329 403-10 (2003)
PMID: 12767825

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cellulase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 22.93 KDa
Source organism: Melanocarpus albomyces
UniProt:
  • Canonical: Q8J0K8 (Residues: 22-235; Coverage: 100%)
Gene name: cel45A
Sequence domains: Glycosyl hydrolase family 45
Structure domains: RlpA-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 47.3Å b: 47.3Å c: 178.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.249

Quick links

Citations

2 review citations

Thermophilic molds: Biology and applications.
Singh et al. (2016)
1 more

1 mention without citation

Cellulases from thermophilic fungi: recent insights and biotechnological potential.
Li et al. (2011)