1oaz

X-ray diffraction
2.78Å resolution

IgE Fv SPE7 complexed with a recombinant thioredoxin

Released:
Source organisms:
Primary publication:
Antibody multispecificity mediated by conformational diversity.
Science 299 1362-7 (2003)
PMID: 12610298

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208469 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thioredoxin 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 13.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 1-35, 36-109; Coverage: 100%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
IMMUNOGLOBULIN E Chains: H, J
Molecule details ›
Chains: H, J
Length: 122 amino acids
Theoretical weight: 13.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig lambda-1 chain V regions MOPC 104E/RPC20/J558/S104 Chains: L, N
Molecule details ›
Chains: L, N
Length: 110 amino acids
Theoretical weight: 11.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01724 (Residues: 20-128; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 79.352Å b: 79.467Å c: 170.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.276
Expression system: Escherichia coli