› 1of3

X-ray diffraction
2Å resolution

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27

Released:
DOI: 10.2210/pdb1of3/pdb
PDB entry 1of3 coloured by chain, front view.
PDB entry 1of3 coloured by chain, side view.
PDB entry 1of3 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27.
Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ
Structure 11 665-75 (2003)
PMID: 12791255

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.63 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9RIK9 (Residues: 505-680; Coverage: 26%)
Gene name: manB
Sequence domains: Carbohydrate binding module 27
Structure domains: Galactose-binding domain-like

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 69.078Å b: 69.078Å c: 229.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
6 more

3 mentions without citation

Crystal structure of the variable domain of the Streptococcus gordonii surface protein SspB.
Forsgren et al. (2009)
2 more