› 1oi2

X-ray diffraction
1.75Å resolution

X-ray structure of the dihydroxyacetone kinase from Escherichia coli

Released:
DOI: 10.2210/pdb1oi2/pdb
PDB entry 1oi2 coloured by chain, front view.
PDB entry 1oi2 coloured by chain, side view.
PDB entry 1oi2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A mechanism of covalent substrate binding in the x-ray structure of subunit K of the Escherichia coli dihydroxyacetone kinase.
Siebold C, García-Alles LF, Erni B, Baumann U
Proc Natl Acad Sci U S A 100 8188-92 (2003)
PMID: 12813127

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 39.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76015 (Residues: 1-356; Coverage: 100%)
Gene names: JW5187, b1200, dhaK, ycgT
Sequence domains: Dak1 domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 96.509Å b: 97.408Å c: 85.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.201
Expression system: Escherichia coli

Quick links

Citations

3 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
2 more

3 mentions without citation

A mechanism of covalent substrate binding in the x-ray structure of subunit K of the Escherichia coli dihydroxyacetone kinase.
Siebold et al. (2003)
2 more