› 1onh

X-ray diffraction
1.38Å resolution

GC1 beta-lactamase with a penem inhibitor

Released:
DOI: 10.2210/pdb1onh/pdb
PDB entry 1onh coloured by chain, front view.
PDB entry 1onh coloured by chain, side view.
PDB entry 1onh coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Inhibition of class A and class C beta-lactamases by penems: crystallographic structures of a novel 1,4-thiazepine intermediate.
Nukaga M, Abe T, Venkatesan AM, Mansour TS, Bonomo RA, Knox JR
Biochemistry 42 13152-9 (2003)
PMID: 14609325

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 39.61 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59401 (Residues: 21-384; Coverage: 100%)
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand WY4 1 x WY4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 76.288Å b: 68.873Å c: 62.014Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.135 0.202
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Current challenges in antimicrobial chemotherapy: focus on ß-lactamase inhibition.
Bebrone et al. (2010)
7 more

3 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
2 more