› 1oo4

Solution NMR

P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr

Released:
DOI: 10.2210/pdb1oo4/pdb
PDB entry 1oo4 coloured by chain, front view.
PDB entry 1oo4 coloured by chain, side view.
PDB entry 1oo4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Nuclear magnetic resonance structure of the P395S mutant of the N-SH2 domain of the p85 subunit of PI3 kinase: an SH2 domain with altered specificity.
Günther UL, Weyrauch B, Zhang X, Schaffhausen B
Biochemistry 42 11120-7 (2003)
PMID: 14503862

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150053 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.86 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23727 (Residues: 321-431; Coverage: 15%)
Gene name: PIK3R1
Sequence domains: SH2 domain
Structure domains: SH2 domain
8-mer peptide from PDGFr Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

Structural basis for a novel intrapeptidyl H-bond and reverse binding of c-Cbl-TKB domain substrates.
Ng et al. (2008)
2 more

2 mentions without citation

The application of FAST-NMR for the identification of novel drug discovery targets.
Powers et al. (2008)
1 more