› 1opm

X-ray diffraction
2.1Å resolution

OXIDIZED (CU2+) PEPTIDYLGLYCINE ALPHA-HYDROXYLATING MONOOXYGENASE (PHM) WITH BOUND SUBSTRATE

Released:
DOI: 10.2210/pdb1opm/pdb
PDB entry 1opm coloured by chain, front view.
PDB entry 1opm coloured by chain, side view.
PDB entry 1opm coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Substrate-mediated electron transfer in peptidylglycine alpha-hydroxylating monooxygenase.
Prigge ST, Kolhekar AS, Eipper BA, Mains RE, Amzel LM
Nat Struct Biol 6 976-83 (1999)
PMID: 10504734

Function and Biology Details

Reactions catalysed: 
[Peptide]-glycine + 2 ascorbate + O(2) = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H(2)O
[Peptide]-(2S)-2-hydroxyglycine = [peptide]-amide + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidylglycine alpha-amidating monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.58 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14925 (Residues: 45-354; Coverage: 33%)
Gene name: Pam
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CU 2 x CU
Ligand AZI 1 x AZI
Ligand NI 1 x NI
Ligand IYG 2 x IYG
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 69.399Å b: 68.84Å c: 81.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.259
Expression system: Cricetulus griseus

Quick links

Citations

11 review citations

Vitamin C: the known and the unknown and Goldilocks.
Padayatty et al. (2016)
10 more

13 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
12 more