› 1osh

X-ray diffraction
1.8Å resolution

A Chemical, Genetic, and Structural Analysis of the nuclear bile acid receptor FXR

Released:
DOI: 10.2210/pdb1osh/pdb
PDB entry 1osh coloured by chain, front view.
PDB entry 1osh coloured by chain, side view.
PDB entry 1osh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A chemical, genetic, and structural analysis of the nuclear bile acid receptor FXR.
Downes M, Verdecia MA, Roecker AJ, Hughes R, Hogenesch JB, Kast-Woelbern HR, Bowman ME, Ferrer JL, Anisfeld AM, Edwards PA, Rosenfeld JM, Alvarez JG, Noel JP, Nicolaou KC, Evans RM
Mol Cell 11 1079-92 (2003)
PMID: 12718892

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bile acid receptor Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 27.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96RI1 (Residues: 257-486; Coverage: 47%)
Gene names: BAR, FXR, HRR1, NR1H4, RIP14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand FEX 1 x FEX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 36.656Å b: 56.776Å c: 117.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.215 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

84 review citations

Intestinal Crosstalk between Bile Acids and Microbiota and Its Impact on Host Metabolism.
Wahlström et al. (2016)
83 more

23 mentions without citation

An updated review on drug-induced cholestasis: mechanisms and investigation of physicochemical properties and pharmacokinetic parameters.
Yang et al. (2013)
22 more