› 1p4f

X-ray diffraction
1.9Å resolution

DEATH ASSOCIATED PROTEIN KINASE CATALYTIC DOMAIN WITH BOUND INHIBITOR FRAGMENT

Released:
DOI: 10.2210/pdb1p4f/pdb
PDB entry 1p4f coloured by chain, front view.
PDB entry 1p4f coloured by chain, side view.
PDB entry 1p4f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An aminopyridazine-based inhibitor of a pro-apoptotic protein kinase attenuates hypoxia-ischemia induced acute brain injury.
Velentza AV, Wainwright MS, Zasadzki M, Mirzoeva S, Schumacher AM, Haiech J, Focia PJ, Egli M, Watterson DM
Bioorg Med Chem Lett 13 3465-70 (2003)
PMID: 14505650

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53355 (Residues: 2-294; Coverage: 21%)
Gene names: DAPK, DAPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 86Q 1 x 86Q
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 47.238Å b: 62.527Å c: 88.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.233
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Death Associated Protein Kinase 1 (DAPK1): A Regulator of Apoptosis and Autophagy.
Singh et al. (2016)
17 more

3 mentions without citation

Opportunities to Target Specific Contractile Abnormalities with Smooth Muscle Protein Kinase Inhibitors.
Ulke-Lemée et al. (2010)
2 more