› 1p8l

X-ray diffraction
2.95Å resolution

New Crystal Structure of Chlorella Virus DNA Ligase-Adenylate

Released:
DOI: 10.2210/pdb1p8l/pdb
PDB entry 1p8l coloured by chain, front view.
PDB entry 1p8l coloured by chain, side view.
PDB entry 1p8l coloured by chain, top view.
Source organism: Paramecium bursaria Chlorella virus 1
Primary publication:
Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate.
Odell M, Malinina L, Sriskanda V, Teplova M, Shuman S
Nucleic Acids Res 31 5090-100 (2003)
PMID: 12930960

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 35.13 KDa
Source organism: Paramecium bursaria Chlorella virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O41026 (Residues: 1-298; Coverage: 100%)
Gene name: A544R
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 91.059Å b: 60.574Å c: 70.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.263 0.304
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Eukaryotic DNA ligases: structural and functional insights.
Ellenberger et al. (2008)
1 more

4 mentions without citation

Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate.
Odell et al. (2003)
3 more