› 1pa7

X-ray diffraction
1.45Å resolution

Crystal structure of amino-terminal microtubule binding domain of EB1

Released:
DOI: 10.2210/pdb1pa7/pdb
PDB entry 1pa7 coloured by chain, front view.
PDB entry 1pa7 coloured by chain, side view.
PDB entry 1pa7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1).
Hayashi I, Ikura M
J Biol Chem 278 36430-4 (2003)
PMID: 12857735

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microtubule-associated protein RP/EB family member 1 Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 15.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15691 (Residues: 1-130; Coverage: 49%)
Gene name: MAPRE1
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 31.856Å b: 48.453Å c: 44.968Å
α: 90° β: 103.36° γ: 90°
R-values:
R R work R free
0.174 0.173 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
Akhmanova et al. (2008)
25 more

11 mentions without citation

Mammalian end binding proteins control persistent microtubule growth.
Komarova et al. (2009)
10 more