› 1pc9

X-ray diffraction
2.5Å resolution

Crystal Structure of BnSP-6, a Lys49-Phospholipase A2

Released:
DOI: 10.2210/pdb1pc9/pdb
PDB entry 1pc9 coloured by chain, front view.
PDB entry 1pc9 coloured by chain, side view.
PDB entry 1pc9 coloured by chain, top view.
Source organism: Bothrops pauloensis
Primary publication:
Crystal structures of BnSP-7 and BnSP-6, two Lys49-phospholipases A(2): quaternary structure and inhibition mechanism insights.
Magro AJ, Soares AM, Giglio JR, Fontes MR
Biochem Biophys Res Commun 311 713-20 (2003)
PMID: 14623331

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 homolog BnSP-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 13.68 KDa
Source organism: Bothrops pauloensis
UniProt:
  • Canonical: Q9IAT9 (Residues: 1-121; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P3121
Unit cell:
a: 57.67Å b: 57.67Å c: 130.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.255

Quick links

Citations

3 review citations

Snake venom Lys49 myotoxins: From phospholipases A(2) to non-enzymatic membrane disruptors.
Lomonte et al. (2012)
2 more

1 mention without citation

PLA2-like proteins myotoxic mechanism: a dynamic model description.
Borges et al. (2017)