› 1pf3

X-ray diffraction
1.5Å resolution

Crystal Structure of the M441L mutant of the multicopper oxidase CueO

Released:
DOI: 10.2210/pdb1pf3/pdb
PDB entry 1pf3 coloured by chain, front view.
PDB entry 1pf3 coloured by chain, side view.
PDB entry 1pf3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO.
Roberts SA, Wildner GF, Grass G, Weichsel A, Ambrus A, Rensing C, Montfort WR
J Biol Chem 278 31958-63 (2003)
PMID: 12794077

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 54.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand C2C 1 x C2C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 50.445Å b: 91.193Å c: 53.905Å
α: 90° β: 102.35° γ: 90°
R-values:
R R work R free
0.193 0.191 0.228
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Copper active sites in biology.
Solomon et al. (2014)
8 more

2 mentions without citation

The Escherichia coli cell division protein and model Tat substrate SufI (FtsP) localizes to the septal ring and has a multicopper oxidase-like structure.
Tarry et al. (2009)
1 more