› 1po0

X-ray diffraction
2.15Å resolution

Crystal structure of ferric citrate transporter FecA in complex with iron-free citrate

Released:
DOI: 10.2210/pdb1po0/pdb
PDB entry 1po0 coloured by chain, front view.
PDB entry 1po0 coloured by chain, side view.
PDB entry 1po0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural evidence for iron-free citrate and ferric citrate binding to the TonB-dependent outer membrane transporter FecA.
Yue WW, Grizot S, Buchanan SK
J Mol Biol 332 353-68 (2003)
PMID: 12948487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(3+) dicitrate transport protein FecA Chain: A
Molecule details ›
Chain: A
Length: 751 amino acids
Theoretical weight: 83.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13036 (Residues: 34-774; Coverage: 100%)
Gene names: JW4251, b4291, fecA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 113.07Å b: 89.31Å c: 95.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.233 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

27 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
26 more

4 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
3 more