› 1pst

X-ray diffraction
3Å resolution

CRYSTALLOGRAPHIC ANALYSES OF SITE-DIRECTED MUTANTS OF THE PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES

Released:
DOI: 10.2210/pdb1pst/pdb
PDB entry 1pst coloured by chain, front view.
PDB entry 1pst coloured by chain, side view.
PDB entry 1pst coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Crystallographic analyses of site-directed mutants of the photosynthetic reaction center from Rhodobacter sphaeroides.
Chirino AJ, Lous EJ, Huber M, Allen JP, Schenck CC, Paddock ML, Feher G, Rees DC
Biochemistry 33 4584-93 (1994)
PMID: 8161514

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 266 amino acids
Theoretical weight: 29.8 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y8 (Residues: 6-271; Coverage: 94%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 296 amino acids
Theoretical weight: 33.18 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y9 (Residues: 7-302; Coverage: 96%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.69 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y7 (Residues: 12-248; Coverage: 91%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand BCL 3 x BCL
Ligand BPH 3 x BPH
Ligand U10 2 x U10
Ligand FE 1 x FE
Ligand CRT 1 x CRT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 138Å b: 77.5Å c: 141.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 not available
Expression system: Not provided

Quick links

Citations

9 review citations

Overexpression of integral membrane proteins for structural studies.
Grisshammer et al. (1995)
8 more

5 mentions without citation

Prediction of water and metal binding sites and their affinities by using the Fold-X force field.
Schymkowitz et al. (2005)
4 more