› 1qq0

X-ray diffraction
1.76Å resolution

COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA

Released:
DOI: 10.2210/pdb1qq0/pdb
PDB entry 1qq0 coloured by chain, front view.
PDB entry 1qq0 coloured by chain, side view.
PDB entry 1qq0 coloured by chain, top view.
Source organism: Methanosarcina thermophila
Primary publication:
A closer look at the active site of gamma-class carbonic anhydrases: high-resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila.
Iverson TM, Alber BE, Kisker C, Ferry JG, Rees DC
Biochemistry 39 9222-31 (2000)
PMID: 10924115

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 26.43 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P40881 (Residues: 1-247; Coverage: 100%)
Gene name: MSTHT_0588
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

1 bound ligand:
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P213
Unit cell:
a: 82.344Å b: 82.344Å c: 82.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.181 0.217
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Carbonic anhydrases: current state of the art, therapeutic applications and future prospects.
Pastorekova et al. (2004)
13 more

3 mentions without citation

Catalytic site identification--a web server to identify catalytic site structural matches throughout PDB.
Kirshner et al. (2013)
2 more