› 1r7m

X-ray diffraction
2.25Å resolution

The homing endonuclease I-SceI bound to its DNA recognition region

Released:
DOI: 10.2210/pdb1r7m/pdb
PDB entry 1r7m coloured by chain, front view.
PDB entry 1r7m coloured by chain, side view.
PDB entry 1r7m coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity.
Moure CM, Gimble FS, Quiocho FA
J Mol Biol 334 685-95 (2003)
PMID: 14636596

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114502 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Intron-encoded endonuclease I-SceI Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 27.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03882 (Residues: 1-235; Coverage: 100%)
Gene names: OMEGA, Q0160, SCEI, SECY
Sequence domains: LAGLIDADG DNA endonuclease family
Structure domains: Homing endonucleases
5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*AP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 25 nucleotides
Theoretical weight: 7.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*TP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 25 nucleotides
Theoretical weight: 7.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 80.6Å b: 90.7Å c: 115.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
12 more

9 mentions without citation

Widespread but Poorly Understood Bacteria: Candidate Phyla Radiation.
Ji et al. (2022)
8 more