› 1r9g

X-ray diffraction
2.5Å resolution

Three-dimensional Structure of YaaE from Bacillus subtilis

Released:
DOI: 10.2210/pdb1r9g/pdb
PDB entry 1r9g coloured by chain, front view.
PDB entry 1r9g coloured by chain, side view.
PDB entry 1r9g coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis.
Bauer JA, Bennett EM, Begley TP, Ealick SE
J Biol Chem 279 2704-11 (2004)
PMID: 14585832

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PdxT Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.92 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P37528 (Residues: 1-196; Coverage: 100%)
Gene names: BSU00120, pdxT, yaaE
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2, APS BEAMLINE 8-BM
Spacegroup: P212121
Unit cell:
a: 45.5Å b: 80.87Å c: 115.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.24 0.279
Expression system: Escherichia coli

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Citations

9 review citations

Conformational changes in ammonia-channeling glutamine amidotransferases.
Mouilleron et al. (2007)
8 more

4 mentions without citation

Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions.
Barra et al. (2021)
3 more