1rb7

X-ray diffraction
2.1Å resolution

Yeast cytosine deaminase crystal form p212121 with sodium acetate.

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Microseed matrix screening to improve crystals of yeast cytosine deaminase.
Acta Crystallogr D Biol Crystallogr 60 601-5 (2004)
PMID: 14993707

Function and Biology Details

Reaction catalysed:
Cytosine + H(2)O = uracil + NH(3)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 17.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12178 (Residues: 1-158; Coverage: 100%)
Gene names: FCY1, YP9499.17, YPR062W
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 54.489Å b: 72.125Å c: 79.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.211
Expression system: Escherichia coli