› 1rf9

X-ray diffraction
1.8Å resolution

Crystal structure of cytochrome P450-cam with a fluorescent probe D-4-AD (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-butyl-amide)

Released:
DOI: 10.2210/pdb1rf9/pdb
PDB entry 1rf9 coloured by chain, front view.
PDB entry 1rf9 coloured by chain, side view.
PDB entry 1rf9 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Conformational states of cytochrome P450cam revealed by trapping of synthetic molecular wires.
Hays AM, Dunn AR, Chiu R, Gray HB, Stout CD, Goodin DB
J Mol Biol 344 455-69 (2004)
PMID: 15522298

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.96 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand DBR 1 x DBR
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 65.126Å b: 75.112Å c: 93.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.227
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cooperative properties of cytochromes P450.
Denisov et al. (2009)
3 more

7 mentions without citation

MOLE 2.0: advanced approach for analysis of biomacromolecular channels.
Sehnal et al. (2013)
6 more