› 1rwr

X-ray diffraction
1.72Å resolution

Crystal structure of filamentous hemagglutinin secretion domain

Released:
DOI: 10.2210/pdb1rwr/pdb
PDB entry 1rwr coloured by chain, front view.
PDB entry 1rwr coloured by chain, side view.
PDB entry 1rwr coloured by chain, top view.
Source organism: Bordetella pertussis
Primary publication:
The crystal structure of filamentous hemagglutinin secretion domain and its implications for the two-partner secretion pathway.
Clantin B, Hodak H, Willery E, Locht C, Jacob-Dubuisson F, Villeret V
Proc Natl Acad Sci U S A 101 6194-9 (2004)
PMID: 15079085

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamentous hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 29.72 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli
UniProt:
  • Canonical: P12255 (Residues: 72-372; Coverage: 8%)
Gene names: BP1879, fhaB
Sequence domains: TPS secretion domain
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 108.98Å b: 43.04Å c: 53.8Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.146 0.144 0.19
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Protein secretion in gram-negative bacteria via the autotransporter pathway.
Dautin et al. (2007)
32 more

9 mentions without citation

Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.
Guérin et al. (2017)
8 more