› 1s7s

X-ray diffraction
1.99Å resolution

Crystal structures of the murine class I major histocompatibility complex H-2Kb in complex with LCMV-derived gp33 index peptide and three of its escape variants

Released:
DOI: 10.2210/pdb1s7s/pdb
PDB entry 1s7s coloured by chain, front view.
PDB entry 1s7s coloured by chain, side view.
PDB entry 1s7s coloured by chain, top view.
Source organisms:
Primary publication:
Determination of structural principles underlying three different modes of lymphocytic choriomeningitis virus escape from CTL recognition.
Velloso LM, Michaëlsson J, Ljunggren HG, Schneider G, Achour A
J Immunol 172 5504-11 (2004)
PMID: 15100292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135045 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01901 (Residues: 22-369; Coverage: 100%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Lymphocytic choriomeningitis virus (strain WE)
Expression system: Not provided
UniProt:
  • Canonical: P07399 (Residues: 33-40; Coverage: 2%)
Gene names: GP-C, GPC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21212
Unit cell:
a: 88.262Å b: 135.555Å c: 45.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.232
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 review citation

Structural aspects of chemical modifications in the MHC-restricted immunopeptidome; Implications for immune recognition.
Sandalova et al. (2022)
27 other articles

2 mentions without citation

Non-Invasive Monitoring of CNS MHC-I Molecules in Ischemic Stroke Mice.
Xia et al. (2017)
1 more