› 1seg

X-ray diffraction
1.3Å resolution

Crystal structure of a toxin chimera between Lqh-alpha-IT from the scorpion Leiurus quinquestriatus hebraeus and AAH2 from Androctonus australis hector

Released:
DOI: 10.2210/pdb1seg/pdb
PDB entry 1seg coloured by chain, front view.
PDB entry 1seg coloured by chain, side view.
PDB entry 1seg coloured by chain, top view.
Source organism: Androctonus australis hector
Primary publication:
Molecular basis of the high insecticidal potency of scorpion alpha-toxins.
Karbat I, Frolow F, Froy O, Gilles N, Cohen L, Turkov M, Gordon D, Gurevitz M
J Biol Chem 279 31679-86 (2004)
PMID: 15133045

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-mammal toxin AaH2 Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.42 KDa
Source organism: Androctonus australis hector
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01484 (Residues: 20-83; Coverage: 97%)
Sequence domains: Scorpion toxin-like domain
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand NO3 2 x NO3
Ligand PPI 1 x PPI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I422
Unit cell:
a: 74.523Å b: 74.523Å c: 56.973Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.178
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Overview of scorpion toxins specific for Na+ channels and related peptides: biodiversity, structure-function relationships and evolution.
Rodríguez de la Vega et al. (2005)
10 more

4 mentions without citation

Scorpion venom components that affect ion-channels function.
Quintero-Hernández et al. (2013)
3 more