› 1sfy

X-ray diffraction
2.55Å resolution

Crystal structure of recombinant Erythrina corallodandron Lectin

Released:
DOI: 10.2210/pdb1sfy/pdb
PDB entry 1sfy coloured by chain, front view.
PDB entry 1sfy coloured by chain, side view.
PDB entry 1sfy coloured by chain, top view.
Source organism: Erythrina corallodendron
Primary publication:
Effect of glycosylation on the structure of Erythrina corallodendron lectin.
Kulkarni KA, Srivastava A, Mitra N, Sharon N, Surolia A, Vijayan M, Suguna K
Proteins 56 821-7 (2004)
PMID: 15281133

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lectin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 239 amino acids
Theoretical weight: 26.27 KDa
Source organism: Erythrina corallodendron
Expression system: Escherichia coli
UniProt:
  • Canonical: P16404 (Residues: 27-265; Coverage: 94%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
2 bound ligands:
Ligand MN 6 x MN
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 87.24Å b: 144.89Å c: 127.66Å
α: 90° β: 93.29° γ: 90°
R-values:
R R work R free
0.18 0.18 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

N-linked oligosaccharides as outfitters for glycoprotein folding, form and function.
Mitra et al. (2006)
1 more

1 mention without citation

Role of glycosylation in structure and stability of Erythrina corallodendron lectin (EcorL): a molecular dynamics study.
Kaushik et al. (2011)