› 1shq

X-ray diffraction
2Å resolution

Crystal structure of shrimp alkaline phosphatase with magnesium in M3

Released:
DOI: 10.2210/pdb1shq/pdb
PDB entry 1shq coloured by chain, front view.
PDB entry 1shq coloured by chain, side view.
PDB entry 1shq coloured by chain, top view.
Source organism: Pandalus borealis
Primary publication:
Ligand-binding and metal-exchange crystallographic studies on shrimp alkaline phosphatase.
de Backer MM, McSweeney S, Lindley PF, Hough E
Acta Crystallogr D Biol Crystallogr 60 1555-61 (2004)
PMID: 15333925

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 53.4 KDa
Source organism: Pandalus borealis
UniProt:
  • Canonical: Q9BHT8 (Residues: 1-475; Coverage: 100%)
Gene name: sap
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand ZN 4 x ZN
Ligand MG 2 x MG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 171.007Å b: 171.007Å c: 84.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.238

Quick links

Citations

1 review citation

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
7 other articles

2 mentions without citation

A novel psychrophilic alkaline phosphatase from the metagenome of tidal flat sediments.
Lee et al. (2015)
1 more