› 1sma

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF A MALTOGENIC AMYLASE

Released:
DOI: 10.2210/pdb1sma/pdb
PDB entry 1sma coloured by chain, front view.
PDB entry 1sma coloured by chain, side view.
PDB entry 1sma coloured by chain, top view.
Source organism: Thermus sp. IM6501
Primary publication:
Crystal structure of a maltogenic amylase provides insights into a catalytic versatility.
Kim JS, Cha SS, Kim HJ, Kim TJ, Ha NC, Oh ST, Cho HS, Cho MJ, Kim MJ, Lee HS, Kim JW, Choi KY, Park KH, Oh BH
J Biol Chem 274 26279-86 (1999)
PMID: 10473583

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 588 amino acids
Theoretical weight: 68.3 KDa
Source organism: Thermus sp. IM6501
UniProt:
  • Canonical: O69007 (Residues: 1-588; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P61
Unit cell:
a: 118.37Å b: 118.37Å c: 266.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.267

Quick links

Citations

5 review citations

Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
MacGregor et al. (2001)
4 more

9 mentions without citation

Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance.
Kim et al. (2003)
8 more