› 1smn

X-ray diffraction
2.04Å resolution

IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS

Released:
DOI: 10.2210/pdb1smn/pdb
PDB entry 1smn coloured by chain, front view.
PDB entry 1smn coloured by chain, side view.
PDB entry 1smn coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis.
Miller MD, Krause KL
Protein Sci 5 24-33 (1996)
PMID: 8771193

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.74 KDa
Source organism: Serratia marcescens
Expression system: Serratia marcescens
UniProt:
  • Canonical: P13717 (Residues: 22-266; Coverage: 100%)
Gene names: nuc, nucA
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 106.7Å b: 74.5Å c: 68.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 not available
Expression system: Serratia marcescens

Quick links

Citations

1 review citation

Serratia marcescens and its extracellular nuclease.
Benedik et al. (1998)
33 other articles

6 mentions without citation

Prediction of catalytic residues using Support Vector Machine with selected protein sequence and structural properties.
Petrova et al. (2006)
5 more