› 1swe

X-ray diffraction
2.06Å resolution

APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN AT PH 4.5

Released:
DOI: 10.2210/pdb1swe/pdb
PDB entry 1swe coloured by chain, front view.
PDB entry 1swe coloured by chain, side view.
PDB entry 1swe coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structural studies of the streptavidin binding loop.
Freitag S, Le Trong I, Klumb L, Stayton PS, Stenkamp RE
Protein Sci 6 1157-66 (1997)
PMID: 9194176

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand BTN 4 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 52.78Å b: 100.11Å c: 52.01Å
α: 90° β: 112.6° γ: 90°
R-values:
R R work R free
0.168 not available 0.266
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Attenuated total reflection infrared spectroscopy of proteins and lipids in biological membranes.
Goormaghtigh et al. (1999)
5 more

18 mentions without citation

Cooperative hydrogen bond interactions in the streptavidin-biotin system.
Hyre et al. (2006)
17 more