› 1t5l

X-ray diffraction
2.6Å resolution

Crystal structure of the DNA repair protein UvrB point mutant Y96A revealing a novel fold for domain 2

Released:
DOI: 10.2210/pdb1t5l/pdb
PDB entry 1t5l coloured by chain, front view.
PDB entry 1t5l coloured by chain, side view.
PDB entry 1t5l coloured by chain, top view.
Source organism: [Bacillus] caldotenax
Primary publication:
Interactions between UvrA and UvrB: the role of UvrB's domain 2 in nucleotide excision repair.
Truglio JJ, Croteau DL, Skorvaga M, DellaVecchia MJ, Theis K, Mandavilli BS, Van Houten B, Kisker C
EMBO J 23 2498-509 (2004)
PMID: 15192705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UvrABC system protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 658 amino acids
Theoretical weight: 75.49 KDa
Source organism: [Bacillus] caldotenax
Expression system: Escherichia coli
UniProt:
  • Canonical: P56981 (Residues: 2-190, 191-658; Coverage: 100%)
Gene name: uvrB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P3221
Unit cell:
a: 150.815Å b: 150.815Å c: 159.832Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.23 0.287
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Repair of UV damage in bacteria.
Goosen et al. (2008)
6 more

7 mentions without citation

Prokaryotic nucleotide excision repair.
Kisker et al. (2013)
6 more