› 1t6m

X-ray diffraction
2.11Å resolution

X-ray Structure of the R70D PI-PLC enzyme: Insight into the role of calcium and surrounding amino acids on active site geometry and catalysis.

Released:
DOI: 10.2210/pdb1t6m/pdb
PDB entry 1t6m coloured by chain, front view.
PDB entry 1t6m coloured by chain, side view.
PDB entry 1t6m coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
X-ray structure of the R69D phosphatidylinositol-specific phospholipase C enzyme: insight into the role of calcium and surrounding amino acids in active site geometry and catalysis.
Apiyo D, Zhao L, Tsai MD, Selby TL
Biochemistry 44 9980-9 (2005)
PMID: 16042375

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.64 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08954 (Residues: 32-329; Coverage: 100%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 91.221Å b: 147.725Å c: 96.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.24
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C.
Shao et al. (2007)
2 more

1 mention without citation

The cation-π box is a specific phosphatidylcholine membrane targeting motif.
Cheng et al. (2013)