› 1ti1

X-ray diffraction
2.6Å resolution

crystal structure of a mutant DsbA

Released:
DOI: 10.2210/pdb1ti1/pdb
PDB entry 1ti1 coloured by chain, front view.
PDB entry 1ti1 coloured by chain, side view.
PDB entry 1ti1 coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Intriguing conformation changes associated with the trans/cis isomerization of a prolyl residue in the active site of the DsbA C33A mutant.
Ondo-Mbele E, Vivès C, Koné A, Serre L
J Mol Biol 347 555-63 (2005)
PMID: 15755450

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.12 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG4 (Residues: 20-208; Coverage: 100%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand D12 1 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P62
Unit cell:
a: 57.16Å b: 57.16Å c: 109.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.279
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The name's bond......disulfide bond.
Heras et al. (2007)
8 other articles

1 mention without citation

The Redox State Regulates the Conformation of Rv2466c to Activate the Antitubercular Prodrug TP053.
Albesa-Jové et al. (2015)