› 1tju

X-ray diffraction
2.1Å resolution

Crystal Structure of T161S Duck Delta 2 Crystallin Mutant

Released:
DOI: 10.2210/pdb1tju/pdb
PDB entry 1tju coloured by chain, front view.
PDB entry 1tju coloured by chain, side view.
PDB entry 1tju coloured by chain, top view.
Source organism: Anas platyrhynchos
Primary publication:
Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.
Sampaleanu LM, Codding PW, Lobsanov YD, Tsai M, Smith GD, Horvatin C, Howell PL
Biochem J 384 437-47 (2004)
PMID: 15320872

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 52.59 KDa
Source organism: Anas platyrhynchos
Expression system: Escherichia coli
UniProt:
  • Canonical: P24058 (Residues: 1-468; Coverage: 100%)
Gene name: ASL2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 93.318Å b: 98.896Å c: 106.996Å
α: 90° β: 101.53° γ: 90°
R-values:
R R work R free
0.196 0.178 0.221
Expression system: Escherichia coli

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Citations

1 review citation

Chemical biology of protein arginine modifications in epigenetic regulation.
Fuhrmann et al. (2015)
9 other articles

2 mentions without citation

Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.
Sampaleanu et al. (2004)
1 more