› 1tjy

X-ray diffraction
1.3Å resolution

Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF

Released:
DOI: 10.2210/pdb1tjy/pdb
PDB entry 1tjy coloured by chain, front view.
PDB entry 1tjy coloured by chain, side view.
PDB entry 1tjy coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2.
Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, Bassler BL, Hughson FM
Mol Cell 15 677-87 (2004)
PMID: 15350213

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187239 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer 2-binding protein LsrB Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.25 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8ZKQ1 (Residues: 27-340; Coverage: 100%)
Gene names: STM4077, lsrB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PAV 1 x PAV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 37.824Å b: 76.623Å c: 109.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.172
Expression system: Escherichia coli BL21

Quick links

Citations

83 review citations

Quorum sensing: cell-to-cell communication in bacteria.
Waters et al. (2005)
82 more

11 mentions without citation

Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes.
Zhang et al. (2020)
10 more