› 1tqx

X-ray diffraction
2Å resolution

Crystal Structure of Pfal009167 A Putative D-Ribulose 5-Phosphate 3-Epimerase from P.falciparum

Released:
DOI: 10.2210/pdb1tqx/pdb
PDB entry 1tqx coloured by chain, front view.
PDB entry 1tqx coloured by chain, side view.
PDB entry 1tqx coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Structure of a ribulose 5-phosphate 3-epimerase from Plasmodium falciparum.
Caruthers J, Bosch J, Buckner F, Van Voorhis W, Myler P, Worthey E, Mehlin C, Boni E, DeTitta G, Luft J, Lauricella A, Kalyuzhniy O, Anderson L, Zucker F, Soltis M, Hol WG
Proteins 62 338-42 (2006)
PMID: 16304640

Function and Biology Details

Reaction catalysed: 
D-ribulose 5-phosphate = D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose-phosphate 3-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.58 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8I5L3 (Residues: 1-227; Coverage: 100%)
Gene name: PF3D7_1219900
Sequence domains: Ribulose-phosphate 3 epimerase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 79.415Å b: 84.454Å c: 120.717Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.233
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Features and applications of microbial sugar epimerases.
Ito S. (2009)
2 more

7 mentions without citation

Evolution of the arginase fold and functional diversity.
Dowling et al. (2008)
6 more