› 1u12

X-ray diffraction
2.7Å resolution

M. loti cyclic nucleotide binding domain mutant

Released:
DOI: 10.2210/pdb1u12/pdb
PDB entry 1u12 coloured by chain, front view.
PDB entry 1u12 coloured by chain, side view.
PDB entry 1u12 coloured by chain, top view.
Source organism: Mesorhizobium japonicum MAFF 303099
Primary publication:
Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel.
Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH
Cell 119 615-27 (2004)
PMID: 15550244

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-gated potassium channel mll3241 Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 14.5 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98GN8 (Residues: 218-355; Coverage: 39%)
Gene name: mll3241
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand IOD 31 x IOD
Ligand SO4 2 x SO4
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 57.126Å b: 57.126Å c: 193.037Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.253 0.281
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Epac and PKA: a tale of two intracellular cAMP receptors.
Cheng et al. (2008)
10 more

6 mentions without citation

Structural basis of properties, mechanisms, and channelopathy of cyclic nucleotide-gated channels.
Hu et al. (2023)
5 more