› 1u18

X-ray diffraction
1.96Å resolution

1.96 A Crystal structure of H60C mutant of nitrophorin complexed with histamine

Released:
DOI: 10.2210/pdb1u18/pdb
PDB entry 1u18 coloured by chain, front view.
PDB entry 1u18 coloured by chain, side view.
PDB entry 1u18 coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Vetter SW, Goodin DB

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.61 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26239 (Residues: 24-207; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand HEM 2 x HEM
Ligand HSM 2 x HSM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 38.82Å b: 73.85Å c: 65.38Å
α: 90° β: 98.57° γ: 90°
R-values:
R R work R free
0.187 0.185 0.226
Expression system: Escherichia coli BL21(DE3)

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