› 1u26

X-ray diffraction
2.5Å resolution

Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate

Released:
DOI: 10.2210/pdb1u26/pdb
PDB entry 1u26 coloured by chain, front view.
PDB entry 1u26 coloured by chain, side view.
PDB entry 1u26 coloured by chain, top view.
Source organism: Selenomonas ruminantium
Primary publication:
Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis.
Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH
Structure 12 2015-24 (2004)
PMID: 15530366

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1L-myo-inositol 1,2,3,4,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine specific protein phosphatases domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 38.59 KDa
Source organism: Selenomonas ruminantium
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7WUJ1 (Residues: 28-346; Coverage: 100%)
Gene name: phyA
Sequence domains: Inositol hexakisphosphate
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IHS 3 x IHS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P21212
Unit cell:
a: 161.301Å b: 101.923Å c: 46.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Phytase, a new life for an "old" enzyme.
Lei et al. (2013)
8 more

4 mentions without citation

Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase.
Puhl et al. (2007)
3 more