› 1uks

X-ray diffraction
1.9Å resolution

Crystal structure of F183L/F259L mutant cyclodextrin glucanotransferase complexed with a pseudo-maltotetraose derived from acarbose

Released:
DOI: 10.2210/pdb1uks/pdb
PDB entry 1uks coloured by chain, front view.
PDB entry 1uks coloured by chain, side view.
PDB entry 1uks coloured by chain, top view.
Source organism: Bacillus sp. 1011
Primary publication:
Effects of essential carbohydrate/aromatic stacking interaction with Tyr100 and Phe259 on substrate binding of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp. 1011.
Haga K, Kanai R, Sakamoto O, Aoyagi M, Harata K, Yamane K
J Biochem 134 881-91 (2003)
PMID: 14769878

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cyclomaltodextrin glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 686 amino acids
Theoretical weight: 75.16 KDa
Source organism: Bacillus sp. 1011
Expression system: Escherichia coli
UniProt:
  • Canonical: P05618 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, GLD
Carbohydrate polymer
3 bound ligands:
Ligand GLC 2 x GLC
Ligand ACI 2 x ACI
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P1
Unit cell:
a: 64.85Å b: 74.53Å c: 78.94Å
α: 85.19° β: 104.86° γ: 101.04°
R-values:
R R work R free
0.165 0.162 0.215
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.
Leemhuis et al. (2010)
1 more

1 mention without citation

Three-Dimensional Molecular Modeling of a Diverse Range of SC Clan Serine Proteases.
Laskar et al. (2012)