› 1un8

X-ray diffraction
2.5Å resolution

Crystal structure of the dihydroxyacetone kinase of C. freundii (native form)

Released:
DOI: 10.2210/pdb1un8/pdb
PDB entry 1un8 coloured by chain, front view.
PDB entry 1un8 coloured by chain, side view.
PDB entry 1un8 coloured by chain, top view.
Source organism: Citrobacter freundii
Primary publication:
Crystal structure of the Citrobacter freundii dihydroxyacetone kinase reveals an eight-stranded alpha-helical barrel ATP-binding domain.
Siebold C, Arnold I, Garcia-Alles LF, Baumann U, Erni B
J Biol Chem 278 48236-44 (2003)
PMID: 12966101

Function and Biology Details

Reaction catalysed: 
ATP + glycerone = ADP + glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155305 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroxyacetone kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 552 amino acids
Theoretical weight: 57.99 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli
UniProt:
  • Canonical: P45510 (Residues: 1-552; Coverage: 100%)
Gene name: dhaK
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand MYY 2 x MYY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: C2221
Unit cell:
a: 100.44Å b: 124.59Å c: 237.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
1 more

4 mentions without citation

Phylogenomic Analyses of Snodgrassella Isolates from Honeybees and Bumblebees Reveal Taxonomic and Functional Diversity.
Cornet et al. (2022)
3 more