› 1up2

X-ray diffraction
1.9Å resolution

Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with glucose-isofagomine at 1.9 angstrom

Released:
DOI: 10.2210/pdb1up2/pdb
PDB entry 1up2 coloured by chain, front view.
PDB entry 1up2 coloured by chain, side view.
PDB entry 1up2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mycobacterium tuberculosis strains possess functional cellulases.
Varrot A, Leydier S, Pell G, Macdonald JM, Stick RV, Henrissat B, Gilbert HJ, Davies GJ
J Biol Chem 280 20181-4 (2005)
PMID: 15824123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 30.58 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q79G13 (Residues: 88-380; Coverage: 77%)
Gene names: Rv0062, cel6, celA, celA1
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

5 bound ligands:
Ligand IFM 2 x IFM
Ligand BGC 2 x BGC
Ligand SO4 2 x SO4
Ligand ACT 2 x ACT
Ligand 1PG 1 x 1PG
1 modified residue:
Ligand MHO 2 x MHO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 61.788Å b: 92.83Å c: 46.695Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.177
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Genome analyses highlight the different biological roles of cellulases.
Mba Medie et al. (2012)
3 more