1urq

X-ray diffraction
2Å resolution

Crystal structure of neuronal Q-SNAREs in complex with R-SNARE motif of Tomosyn

Released:
Source organism: Rattus norvegicus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152476 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Syntaxin-binding protein 5 Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 6.69 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WU70 (Residues: 1086-1145; Coverage: 5%)
Gene names: Llgl3, Stxbp5
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 8.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32851 (Residues: 185-259; Coverage: 26%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 9.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60881 (Residues: 5-83; Coverage: 38%)
  • Best match: P60881-2 (Residues: 5-83)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60881 (Residues: 141-204; Coverage: 31%)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2
Unit cell:
a: 126.054Å b: 35.602Å c: 93.541Å
α: 90° β: 132.38° γ: 90°
R-values:
R R work R free
0.166 0.165 0.224
Expression system: Escherichia coli BL21(DE3)