› 1uut

X-ray diffraction
2Å resolution

The Nuclease Domain of Adeno-Associated Virus Rep Complexed with the RBE' Stemloop of the Viral Inverted Terminal Repeat

Released:
DOI: 10.2210/pdb1uut/pdb
PDB entry 1uut coloured by chain, front view.
PDB entry 1uut coloured by chain, side view.
PDB entry 1uut coloured by chain, top view.
Source organism: adeno-associated virus 5
Primary publication:
The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces.
Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F
Mol Cell 13 403-14 (2004)
PMID: 14967147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114711 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Rep protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 22.78 KDa
Source organism: adeno-associated virus 5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9YJC1 (Residues: 1-197; Coverage: 32%)
Gene names: Rep22, rep
Sequence domains: Rep protein catalytic domain like
Structure domains: Replication Protein E1; Chain: A,
5'-D(*CP*AP*GP*CP*TP*CP*TP*TP*TP*GP *AP*GP*CP*TP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: adeno-associated virus 5
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 41.83Å b: 78.705Å c: 184.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Biological diversity of prokaryotic type IV secretion systems.
Alvarez-Martinez et al. (2009)
8 more

7 mentions without citation

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
6 more