› 1v14

X-ray diffraction
2.9Å resolution

Crystal Structure of the Colicin E9, mutant His103Ala, in complex with Mg+2 and dsDNA (resolution 2.9A)

Released:
DOI: 10.2210/pdb1v14/pdb
PDB entry 1v14 coloured by chain, front view.
PDB entry 1v14 coloured by chain, side view.
PDB entry 1v14 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-based analysis of the metal-dependent mechanism of H-N-H endonucleases.
Maté MJ, Kleanthous C
J Biol Chem 279 34763-9 (2004)
PMID: 15190054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114712 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Colicin-E9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 15.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain
5'-D(*GP*CP*GP*AP*TP*CP*GP*CP)-3' Chains: E, F, G, H, I, J, K, L
Molecule details ›
Chains: E, F, G, H, I, J, K, L
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 92.946Å b: 124.442Å c: 111.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.306
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
2 more

4 mentions without citation

Colicin biology.
Cascales et al. (2007)
3 more