› 1v34

X-ray diffraction
2.7Å resolution

Crystal structure of Pyrococcus horikoshii DNA primase-UTP complex

Released:
DOI: 10.2210/pdb1v34/pdb
Model geometry
Fit model/data
PDB entry 1v34 coloured by chain, front view.
PDB entry 1v34 coloured by chain, side view.
PDB entry 1v34 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis.
Ito N, Nureki O, Shirouzu M, Yokoyama S, Hanaoka F
Genes Cells 8 913-23 (2003)
PMID: 14750947

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129634 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase small subunit PriS Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 42.64 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57934 (Residues: 1-346; Coverage: 100%)
Gene names: PH0195, priA, priS
Sequence domains: DNA primase small subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand UTP 1 x UTP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P3221
Unit cell:
a: 76.386Å b: 76.386Å c: 128.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.262
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial DNA repair by non-homologous end joining.
Shuman et al. (2007)
4 more

7 mentions without citation

Mechanism and evolution of DNA primases.
Kuchta et al. (2010)
6 more