› 1v9l

X-ray diffraction
2.8Å resolution

L-glutamate dehydrogenase from Pyrobaculum islandicum complexed with NAD

Released:
DOI: 10.2210/pdb1v9l/pdb
PDB entry 1v9l coloured by chain, front view.
PDB entry 1v9l coloured by chain, side view.
PDB entry 1v9l coloured by chain, top view.
Source organism: Pyrobaculum islandicum
Primary publication:
The first crystal structure of hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum.
Bhuiya MW, Sakuraba H, Ohshima T, Imagawa T, Katunuma N, Tsuge H
J Mol Biol 345 325-37 (2005)
PMID: 15571725

Function and Biology Details

Reaction catalysed: 
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 421 amino acids
Theoretical weight: 46.96 KDa
Source organism: Pyrobaculum islandicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y8I4 (Residues: 1-421; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 104.21Å b: 165.88Å c: 181.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.2 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Enzymatic asymmetric synthesis of chiral amino acids.
Xue et al. (2018)
5 more

4 mentions without citation

The interface of protein structure, protein biophysics, and molecular evolution.
Liberles et al. (2012)
3 more