› 1vbi

X-ray diffraction
1.8Å resolution

Crystal structure of type 2 malate/lactate dehydrogenase from thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1vbi/pdb
PDB entry 1vbi coloured by chain, front view.
PDB entry 1vbi coloured by chain, side view.
PDB entry 1vbi coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate/L-lactate dehydrogenase family protein Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 37 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53W84 (Residues: 1-344; Coverage: 100%)
Gene name: TTHB078
Sequence domains: Malate/L-lactate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P6522
Unit cell:
a: 78.32Å b: 78.32Å c: 253.213Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links