1ve3

X-ray diffraction
2.1Å resolution

Crystal structure of PH0226 protein from Pyrococcus horikoshii OT3

Released:
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of SAM-dependent methyltransferase from Pyrococcus horikoshii.
Acta Crystallogr F Struct Biol Commun 73 706-712 (2017)
PMID: 29199993

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-129646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 27.06 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O57965 (Residues: 1-227; Coverage: 100%)
Gene name: PH0226
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P3221
Unit cell:
a: 58.167Å b: 58.167Å c: 252.463Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.27
Expression system: Escherichia coli BL21(DE3)