› 1vg0

X-ray diffraction
2.2Å resolution

The crystal structures of the REP-1 protein in complex with monoprenylated Rab7 protein

Released:
DOI: 10.2210/pdb1vg0/pdb
PDB entry 1vg0 coloured by chain, front view.
PDB entry 1vg0 coloured by chain, side view.
PDB entry 1vg0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease.
Rak A, Pylypenko O, Niculae A, Pyatkov K, Goody RS, Alexandrov K
Cell 117 749-60 (2004)
PMID: 15186776

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140685 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rab proteins geranylgeranyltransferase component A 1 Chain: A
Molecule details ›
Chain: A
Length: 650 amino acids
Theoretical weight: 72.62 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P37727 (Residues: 1-650; Coverage: 100%)
Gene names: Chm, Rep1
Sequence domains:
Structure domains:
Ras-related protein Rab-7a Chain: B
Molecule details ›
Chain: B
Length: 207 amino acids
Theoretical weight: 23.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09527 (Residues: 1-207; Coverage: 100%)
Gene names: Rab7, Rab7a
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand CL 1 x CL
Ligand GER 1 x GER
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 64.3Å b: 105.3Å c: 132.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.233
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Role of Rab GTPases in membrane traffic and cell physiology.
Hutagalung et al. (2011)
23 more

18 mentions without citation

Molecular control of Rab activity by GEFs, GAPs and GDI.
Müller et al. (2018)
17 more